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goat anti cd36 antibody  (R&D Systems)


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    R&D Systems goat anti cd36 antibody
    Goat Anti Cd36 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti-cd36/Human+CD36%2FSR-B3+Antibody/pm40683443-76-24-29
    Average 92 stars, based on 16 article reviews
    goat anti cd36 antibody - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Incubation:

    Article Title: Nalmefene, an opioid receptor modulator, aggravates atherosclerotic plaque formation in apolipoprotein E knockout mice by enhancing oxidized low-density lipoprotein uptake in macrophages
    Article Snippet: The blot was incubated with goat anti-CD36 (1:500; R&D Systems, Minneapolis, MN, USA) and mouse anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 1:2000; Millipore) antibodies at 4 °C overnight, and then incubated with horseradish peroxidase-conjugated anti-goat and -mouse (1:10000 dilution, Bio-Rad Laboratories, Hercules, CA, USA) antibodies for 60 min at room temperature.

    Article Title: Regulation of Insulin Receptor Pathway and Glucose Metabolism by CD36 Signaling
    Article Snippet: After incubation for 16 h with goat anti-CD36 (R&D Systems) and rabbit anti-IRβ (Cell Signaling) antibodies, processing followed the manufacturers’ instructions.

    Expressing:

    Article Title: Nalmefene, an opioid receptor modulator, aggravates atherosclerotic plaque formation in apolipoprotein E knockout mice by enhancing oxidized low-density lipoprotein uptake in macrophages
    Article Snippet: The blot was incubated with goat anti-CD36 (1:500; R&D Systems, Minneapolis, MN, USA) and mouse anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 1:2000; Millipore) antibodies at 4 °C overnight, and then incubated with horseradish peroxidase-conjugated anti-goat and -mouse (1:10000 dilution, Bio-Rad Laboratories, Hercules, CA, USA) antibodies for 60 min at room temperature.

    Article Title: Regulation of Insulin Receptor Pathway and Glucose Metabolism by CD36 Signaling
    Article Snippet: After incubation for 16 h with goat anti-CD36 (R&D Systems) and rabbit anti-IRβ (Cell Signaling) antibodies, processing followed the manufacturers’ instructions.

    Western Blot:

    Article Title: Nalmefene, an opioid receptor modulator, aggravates atherosclerotic plaque formation in apolipoprotein E knockout mice by enhancing oxidized low-density lipoprotein uptake in macrophages
    Article Snippet: The blot was incubated with goat anti-CD36 (1:500; R&D Systems, Minneapolis, MN, USA) and mouse anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 1:2000; Millipore) antibodies at 4 °C overnight, and then incubated with horseradish peroxidase-conjugated anti-goat and -mouse (1:10000 dilution, Bio-Rad Laboratories, Hercules, CA, USA) antibodies for 60 min at room temperature.

    Article Title: Regulation of Insulin Receptor Pathway and Glucose Metabolism by CD36 Signaling
    Article Snippet: After incubation for 16 h with goat anti-CD36 (R&D Systems) and rabbit anti-IRβ (Cell Signaling) antibodies, processing followed the manufacturers’ instructions.

    Real-time Polymerase Chain Reaction:

    Article Title: Nalmefene, an opioid receptor modulator, aggravates atherosclerotic plaque formation in apolipoprotein E knockout mice by enhancing oxidized low-density lipoprotein uptake in macrophages
    Article Snippet: The blot was incubated with goat anti-CD36 (1:500; R&D Systems, Minneapolis, MN, USA) and mouse anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 1:2000; Millipore) antibodies at 4 °C overnight, and then incubated with horseradish peroxidase-conjugated anti-goat and -mouse (1:10000 dilution, Bio-Rad Laboratories, Hercules, CA, USA) antibodies for 60 min at room temperature.

    Article Title: Regulation of Insulin Receptor Pathway and Glucose Metabolism by CD36 Signaling
    Article Snippet: After incubation for 16 h with goat anti-CD36 (R&D Systems) and rabbit anti-IRβ (Cell Signaling) antibodies, processing followed the manufacturers’ instructions.

    Immunostaining:

    Article Title: Nalmefene, an opioid receptor modulator, aggravates atherosclerotic plaque formation in apolipoprotein E knockout mice by enhancing oxidized low-density lipoprotein uptake in macrophages
    Article Snippet: The blot was incubated with goat anti-CD36 (1:500; R&D Systems, Minneapolis, MN, USA) and mouse anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 1:2000; Millipore) antibodies at 4 °C overnight, and then incubated with horseradish peroxidase-conjugated anti-goat and -mouse (1:10000 dilution, Bio-Rad Laboratories, Hercules, CA, USA) antibodies for 60 min at room temperature.

    Article Title: Regulation of Insulin Receptor Pathway and Glucose Metabolism by CD36 Signaling
    Article Snippet: After incubation for 16 h with goat anti-CD36 (R&D Systems) and rabbit anti-IRβ (Cell Signaling) antibodies, processing followed the manufacturers’ instructions.

    Staining:

    Article Title: Nalmefene, an opioid receptor modulator, aggravates atherosclerotic plaque formation in apolipoprotein E knockout mice by enhancing oxidized low-density lipoprotein uptake in macrophages
    Article Snippet: The blot was incubated with goat anti-CD36 (1:500; R&D Systems, Minneapolis, MN, USA) and mouse anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 1:2000; Millipore) antibodies at 4 °C overnight, and then incubated with horseradish peroxidase-conjugated anti-goat and -mouse (1:10000 dilution, Bio-Rad Laboratories, Hercules, CA, USA) antibodies for 60 min at room temperature.

    Article Title: Regulation of Insulin Receptor Pathway and Glucose Metabolism by CD36 Signaling
    Article Snippet: After incubation for 16 h with goat anti-CD36 (R&D Systems) and rabbit anti-IRβ (Cell Signaling) antibodies, processing followed the manufacturers’ instructions.

    In Vivo:

    Article Title: Nalmefene, an opioid receptor modulator, aggravates atherosclerotic plaque formation in apolipoprotein E knockout mice by enhancing oxidized low-density lipoprotein uptake in macrophages
    Article Snippet: The blot was incubated with goat anti-CD36 (1:500; R&D Systems, Minneapolis, MN, USA) and mouse anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 1:2000; Millipore) antibodies at 4 °C overnight, and then incubated with horseradish peroxidase-conjugated anti-goat and -mouse (1:10000 dilution, Bio-Rad Laboratories, Hercules, CA, USA) antibodies for 60 min at room temperature.

    Article Title: Regulation of Insulin Receptor Pathway and Glucose Metabolism by CD36 Signaling
    Article Snippet: After incubation for 16 h with goat anti-CD36 (R&D Systems) and rabbit anti-IRβ (Cell Signaling) antibodies, processing followed the manufacturers’ instructions.

    Binding Assay:

    Article Title: Nalmefene, an opioid receptor modulator, aggravates atherosclerotic plaque formation in apolipoprotein E knockout mice by enhancing oxidized low-density lipoprotein uptake in macrophages
    Article Snippet: The blot was incubated with goat anti-CD36 (1:500; R&D Systems, Minneapolis, MN, USA) and mouse anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 1:2000; Millipore) antibodies at 4 °C overnight, and then incubated with horseradish peroxidase-conjugated anti-goat and -mouse (1:10000 dilution, Bio-Rad Laboratories, Hercules, CA, USA) antibodies for 60 min at room temperature.

    Article Title: Regulation of Insulin Receptor Pathway and Glucose Metabolism by CD36 Signaling
    Article Snippet: After incubation for 16 h with goat anti-CD36 (R&D Systems) and rabbit anti-IRβ (Cell Signaling) antibodies, processing followed the manufacturers’ instructions.

    Injection:

    Article Title: Nalmefene, an opioid receptor modulator, aggravates atherosclerotic plaque formation in apolipoprotein E knockout mice by enhancing oxidized low-density lipoprotein uptake in macrophages
    Article Snippet: The blot was incubated with goat anti-CD36 (1:500; R&D Systems, Minneapolis, MN, USA) and mouse anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 1:2000; Millipore) antibodies at 4 °C overnight, and then incubated with horseradish peroxidase-conjugated anti-goat and -mouse (1:10000 dilution, Bio-Rad Laboratories, Hercules, CA, USA) antibodies for 60 min at room temperature.

    Article Title: Regulation of Insulin Receptor Pathway and Glucose Metabolism by CD36 Signaling
    Article Snippet: After incubation for 16 h with goat anti-CD36 (R&D Systems) and rabbit anti-IRβ (Cell Signaling) antibodies, processing followed the manufacturers’ instructions.

    Membrane:

    Article Title: Nalmefene, an opioid receptor modulator, aggravates atherosclerotic plaque formation in apolipoprotein E knockout mice by enhancing oxidized low-density lipoprotein uptake in macrophages
    Article Snippet: The blot was incubated with goat anti-CD36 (1:500; R&D Systems, Minneapolis, MN, USA) and mouse anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 1:2000; Millipore) antibodies at 4 °C overnight, and then incubated with horseradish peroxidase-conjugated anti-goat and -mouse (1:10000 dilution, Bio-Rad Laboratories, Hercules, CA, USA) antibodies for 60 min at room temperature.

    Article Title: Regulation of Insulin Receptor Pathway and Glucose Metabolism by CD36 Signaling
    Article Snippet: After incubation for 16 h with goat anti-CD36 (R&D Systems) and rabbit anti-IRβ (Cell Signaling) antibodies, processing followed the manufacturers’ instructions.

    Translocation Assay:

    Article Title: Nalmefene, an opioid receptor modulator, aggravates atherosclerotic plaque formation in apolipoprotein E knockout mice by enhancing oxidized low-density lipoprotein uptake in macrophages
    Article Snippet: The blot was incubated with goat anti-CD36 (1:500; R&D Systems, Minneapolis, MN, USA) and mouse anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 1:2000; Millipore) antibodies at 4 °C overnight, and then incubated with horseradish peroxidase-conjugated anti-goat and -mouse (1:10000 dilution, Bio-Rad Laboratories, Hercules, CA, USA) antibodies for 60 min at room temperature.

    Article Title: Regulation of Insulin Receptor Pathway and Glucose Metabolism by CD36 Signaling
    Article Snippet: After incubation for 16 h with goat anti-CD36 (R&D Systems) and rabbit anti-IRβ (Cell Signaling) antibodies, processing followed the manufacturers’ instructions.

    Inhibition:

    Article Title: Nalmefene, an opioid receptor modulator, aggravates atherosclerotic plaque formation in apolipoprotein E knockout mice by enhancing oxidized low-density lipoprotein uptake in macrophages
    Article Snippet: The blot was incubated with goat anti-CD36 (1:500; R&D Systems, Minneapolis, MN, USA) and mouse anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 1:2000; Millipore) antibodies at 4 °C overnight, and then incubated with horseradish peroxidase-conjugated anti-goat and -mouse (1:10000 dilution, Bio-Rad Laboratories, Hercules, CA, USA) antibodies for 60 min at room temperature.

    Article Title: Regulation of Insulin Receptor Pathway and Glucose Metabolism by CD36 Signaling
    Article Snippet: After incubation for 16 h with goat anti-CD36 (R&D Systems) and rabbit anti-IRβ (Cell Signaling) antibodies, processing followed the manufacturers’ instructions.

    Plasmid Preparation:

    Article Title: Nalmefene, an opioid receptor modulator, aggravates atherosclerotic plaque formation in apolipoprotein E knockout mice by enhancing oxidized low-density lipoprotein uptake in macrophages
    Article Snippet: The blot was incubated with goat anti-CD36 (1:500; R&D Systems, Minneapolis, MN, USA) and mouse anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 1:2000; Millipore) antibodies at 4 °C overnight, and then incubated with horseradish peroxidase-conjugated anti-goat and -mouse (1:10000 dilution, Bio-Rad Laboratories, Hercules, CA, USA) antibodies for 60 min at room temperature.

    Article Title: Regulation of Insulin Receptor Pathway and Glucose Metabolism by CD36 Signaling
    Article Snippet: After incubation for 16 h with goat anti-CD36 (R&D Systems) and rabbit anti-IRβ (Cell Signaling) antibodies, processing followed the manufacturers’ instructions.

    Activation Assay:

    Article Title: Nalmefene, an opioid receptor modulator, aggravates atherosclerotic plaque formation in apolipoprotein E knockout mice by enhancing oxidized low-density lipoprotein uptake in macrophages
    Article Snippet: The blot was incubated with goat anti-CD36 (1:500; R&D Systems, Minneapolis, MN, USA) and mouse anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 1:2000; Millipore) antibodies at 4 °C overnight, and then incubated with horseradish peroxidase-conjugated anti-goat and -mouse (1:10000 dilution, Bio-Rad Laboratories, Hercules, CA, USA) antibodies for 60 min at room temperature.

    Article Title: Regulation of Insulin Receptor Pathway and Glucose Metabolism by CD36 Signaling
    Article Snippet: After incubation for 16 h with goat anti-CD36 (R&D Systems) and rabbit anti-IRβ (Cell Signaling) antibodies, processing followed the manufacturers’ instructions.

    Co-Immunoprecipitation Assay:

    Article Title: Nalmefene, an opioid receptor modulator, aggravates atherosclerotic plaque formation in apolipoprotein E knockout mice by enhancing oxidized low-density lipoprotein uptake in macrophages
    Article Snippet: The blot was incubated with goat anti-CD36 (1:500; R&D Systems, Minneapolis, MN, USA) and mouse anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 1:2000; Millipore) antibodies at 4 °C overnight, and then incubated with horseradish peroxidase-conjugated anti-goat and -mouse (1:10000 dilution, Bio-Rad Laboratories, Hercules, CA, USA) antibodies for 60 min at room temperature.

    Article Title: Regulation of Insulin Receptor Pathway and Glucose Metabolism by CD36 Signaling
    Article Snippet: After incubation for 16 h with goat anti-CD36 (R&D Systems) and rabbit anti-IRβ (Cell Signaling) antibodies, processing followed the manufacturers’ instructions.

    Negative Control:

    Article Title: Nalmefene, an opioid receptor modulator, aggravates atherosclerotic plaque formation in apolipoprotein E knockout mice by enhancing oxidized low-density lipoprotein uptake in macrophages
    Article Snippet: The blot was incubated with goat anti-CD36 (1:500; R&D Systems, Minneapolis, MN, USA) and mouse anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 1:2000; Millipore) antibodies at 4 °C overnight, and then incubated with horseradish peroxidase-conjugated anti-goat and -mouse (1:10000 dilution, Bio-Rad Laboratories, Hercules, CA, USA) antibodies for 60 min at room temperature.

    Article Title: Regulation of Insulin Receptor Pathway and Glucose Metabolism by CD36 Signaling
    Article Snippet: After incubation for 16 h with goat anti-CD36 (R&D Systems) and rabbit anti-IRβ (Cell Signaling) antibodies, processing followed the manufacturers’ instructions.

    Muscles:

    Article Title: Nalmefene, an opioid receptor modulator, aggravates atherosclerotic plaque formation in apolipoprotein E knockout mice by enhancing oxidized low-density lipoprotein uptake in macrophages
    Article Snippet: The blot was incubated with goat anti-CD36 (1:500; R&D Systems, Minneapolis, MN, USA) and mouse anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 1:2000; Millipore) antibodies at 4 °C overnight, and then incubated with horseradish peroxidase-conjugated anti-goat and -mouse (1:10000 dilution, Bio-Rad Laboratories, Hercules, CA, USA) antibodies for 60 min at room temperature.

    Article Title: Regulation of Insulin Receptor Pathway and Glucose Metabolism by CD36 Signaling
    Article Snippet: After incubation for 16 h with goat anti-CD36 (R&D Systems) and rabbit anti-IRβ (Cell Signaling) antibodies, processing followed the manufacturers’ instructions.

    SDS Page:

    Article Title: Nalmefene, an opioid receptor modulator, aggravates atherosclerotic plaque formation in apolipoprotein E knockout mice by enhancing oxidized low-density lipoprotein uptake in macrophages
    Article Snippet: The blot was incubated with goat anti-CD36 (1:500; R&D Systems, Minneapolis, MN, USA) and mouse anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 1:2000; Millipore) antibodies at 4 °C overnight, and then incubated with horseradish peroxidase-conjugated anti-goat and -mouse (1:10000 dilution, Bio-Rad Laboratories, Hercules, CA, USA) antibodies for 60 min at room temperature.

    Article Title: Regulation of Insulin Receptor Pathway and Glucose Metabolism by CD36 Signaling
    Article Snippet: After incubation for 16 h with goat anti-CD36 (R&D Systems) and rabbit anti-IRβ (Cell Signaling) antibodies, processing followed the manufacturers’ instructions.

    Proximity Ligation Assay:

    Article Title: Nalmefene, an opioid receptor modulator, aggravates atherosclerotic plaque formation in apolipoprotein E knockout mice by enhancing oxidized low-density lipoprotein uptake in macrophages
    Article Snippet: The blot was incubated with goat anti-CD36 (1:500; R&D Systems, Minneapolis, MN, USA) and mouse anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 1:2000; Millipore) antibodies at 4 °C overnight, and then incubated with horseradish peroxidase-conjugated anti-goat and -mouse (1:10000 dilution, Bio-Rad Laboratories, Hercules, CA, USA) antibodies for 60 min at room temperature.

    Article Title: Regulation of Insulin Receptor Pathway and Glucose Metabolism by CD36 Signaling
    Article Snippet: After incubation for 16 h with goat anti-CD36 (R&D Systems) and rabbit anti-IRβ (Cell Signaling) antibodies, processing followed the manufacturers’ instructions.

    Amplification:

    Article Title: Nalmefene, an opioid receptor modulator, aggravates atherosclerotic plaque formation in apolipoprotein E knockout mice by enhancing oxidized low-density lipoprotein uptake in macrophages
    Article Snippet: The blot was incubated with goat anti-CD36 (1:500; R&D Systems, Minneapolis, MN, USA) and mouse anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 1:2000; Millipore) antibodies at 4 °C overnight, and then incubated with horseradish peroxidase-conjugated anti-goat and -mouse (1:10000 dilution, Bio-Rad Laboratories, Hercules, CA, USA) antibodies for 60 min at room temperature.

    Article Title: Regulation of Insulin Receptor Pathway and Glucose Metabolism by CD36 Signaling
    Article Snippet: After incubation for 16 h with goat anti-CD36 (R&D Systems) and rabbit anti-IRβ (Cell Signaling) antibodies, processing followed the manufacturers’ instructions.

    Transfection:

    Article Title: Nalmefene, an opioid receptor modulator, aggravates atherosclerotic plaque formation in apolipoprotein E knockout mice by enhancing oxidized low-density lipoprotein uptake in macrophages
    Article Snippet: The blot was incubated with goat anti-CD36 (1:500; R&D Systems, Minneapolis, MN, USA) and mouse anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 1:2000; Millipore) antibodies at 4 °C overnight, and then incubated with horseradish peroxidase-conjugated anti-goat and -mouse (1:10000 dilution, Bio-Rad Laboratories, Hercules, CA, USA) antibodies for 60 min at room temperature.

    Article Title: Regulation of Insulin Receptor Pathway and Glucose Metabolism by CD36 Signaling
    Article Snippet: After incubation for 16 h with goat anti-CD36 (R&D Systems) and rabbit anti-IRβ (Cell Signaling) antibodies, processing followed the manufacturers’ instructions.

    Mutagenesis:

    Article Title: Nalmefene, an opioid receptor modulator, aggravates atherosclerotic plaque formation in apolipoprotein E knockout mice by enhancing oxidized low-density lipoprotein uptake in macrophages
    Article Snippet: The blot was incubated with goat anti-CD36 (1:500; R&D Systems, Minneapolis, MN, USA) and mouse anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 1:2000; Millipore) antibodies at 4 °C overnight, and then incubated with horseradish peroxidase-conjugated anti-goat and -mouse (1:10000 dilution, Bio-Rad Laboratories, Hercules, CA, USA) antibodies for 60 min at room temperature.

    Article Title: Regulation of Insulin Receptor Pathway and Glucose Metabolism by CD36 Signaling
    Article Snippet: After incubation for 16 h with goat anti-CD36 (R&D Systems) and rabbit anti-IRβ (Cell Signaling) antibodies, processing followed the manufacturers’ instructions.



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    Mice that lack <t>CD36-expressing</t> B cells exhibit compromised autoimmunity. A The immunization strategy employed for the Mb1 cre and Cd36 fl/fl Mb1 cre mice. Mice were immunized four times weekly with apoptotic cells (4xAC), while PBS was used as the negative control. B The gating strategy and frequency of germinal center (GC) B cells were examined. C Measurement of anti-DNA IgG levels via ELISA. The data are representative of three independent experiments. * P < 0.05, ** P < 0.01 and *** P < 0.001 (Mann–Whitney)
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    Image Search Results


    Mice that lack CD36-expressing B cells exhibit compromised autoimmunity. A The immunization strategy employed for the Mb1 cre and Cd36 fl/fl Mb1 cre mice. Mice were immunized four times weekly with apoptotic cells (4xAC), while PBS was used as the negative control. B The gating strategy and frequency of germinal center (GC) B cells were examined. C Measurement of anti-DNA IgG levels via ELISA. The data are representative of three independent experiments. * P < 0.05, ** P < 0.01 and *** P < 0.001 (Mann–Whitney)

    Journal: Cellular & Molecular Biology Letters

    Article Title: Unveiling the hidden role of the interaction between CD36 and FcγRIIb: implications for autoimmune disorders

    doi: 10.1186/s11658-024-00593-7

    Figure Lengend Snippet: Mice that lack CD36-expressing B cells exhibit compromised autoimmunity. A The immunization strategy employed for the Mb1 cre and Cd36 fl/fl Mb1 cre mice. Mice were immunized four times weekly with apoptotic cells (4xAC), while PBS was used as the negative control. B The gating strategy and frequency of germinal center (GC) B cells were examined. C Measurement of anti-DNA IgG levels via ELISA. The data are representative of three independent experiments. * P < 0.05, ** P < 0.01 and *** P < 0.001 (Mann–Whitney)

    Article Snippet: To conduct immunoprecipitation, a total of 2 µg of goat anti-mouse CD36 antibody (R&D) or normal goat IgG (Millipore) was incubated with Pierce™ Protein A/G Magnetic Beads (Thermo Scientific™) overnight.

    Techniques: Expressing, Negative Control, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

    CD36-associated proteins were identified through co-immunoprecipitation followed by mass spectrometry analysis. A The pseudocolor plot illustrates the staining of CD36 in B cells that were either stimulated with CPG (CPG Stim) or unstimulated (Uns). B Confocal microscopy was used to analyze CD36 staining in B cells treated with CPG. C Immunoblot analysis of soluble proteins derived from B cells stimulated with CPG was subsequently performed using an anti-CD36 antibody. The total B cell protein served as the loading (positive) control referred to as Input (Inp). The B cell protein was immunoprecipitated using isotype (negative) control IgG antibodies (IgG) or anti-CD36 antibodies (CD36). D Evidence of the CD36 protein network predicted by the STRING, demonstrating its functional association with the top ten proteins. Network nodes represent proteins. Colored nodes represent the query protein and its first shell of interactors, while white nodes represent the query protein and the second shell of interactors. Filled nodes or empty nodes indicate predicted or unpredicted 3D structures, and edges represent protein–protein associations. Line color indicates the different types of interaction evidence. E Confidence view of the CD36 protein network. The line of thickness visually depicts stronger associations. F , G Gene Ontology enrichment analysis was also conducted to investigate the biological processes and molecular functions associated with the proteins identified through anti-CD36 immunoprecipitation. The data are representative of three independent experiments. * P < 0.05, ** P < 0.01 and *** P < 0.001 (Mann–Whitney)

    Journal: Cellular & Molecular Biology Letters

    Article Title: Unveiling the hidden role of the interaction between CD36 and FcγRIIb: implications for autoimmune disorders

    doi: 10.1186/s11658-024-00593-7

    Figure Lengend Snippet: CD36-associated proteins were identified through co-immunoprecipitation followed by mass spectrometry analysis. A The pseudocolor plot illustrates the staining of CD36 in B cells that were either stimulated with CPG (CPG Stim) or unstimulated (Uns). B Confocal microscopy was used to analyze CD36 staining in B cells treated with CPG. C Immunoblot analysis of soluble proteins derived from B cells stimulated with CPG was subsequently performed using an anti-CD36 antibody. The total B cell protein served as the loading (positive) control referred to as Input (Inp). The B cell protein was immunoprecipitated using isotype (negative) control IgG antibodies (IgG) or anti-CD36 antibodies (CD36). D Evidence of the CD36 protein network predicted by the STRING, demonstrating its functional association with the top ten proteins. Network nodes represent proteins. Colored nodes represent the query protein and its first shell of interactors, while white nodes represent the query protein and the second shell of interactors. Filled nodes or empty nodes indicate predicted or unpredicted 3D structures, and edges represent protein–protein associations. Line color indicates the different types of interaction evidence. E Confidence view of the CD36 protein network. The line of thickness visually depicts stronger associations. F , G Gene Ontology enrichment analysis was also conducted to investigate the biological processes and molecular functions associated with the proteins identified through anti-CD36 immunoprecipitation. The data are representative of three independent experiments. * P < 0.05, ** P < 0.01 and *** P < 0.001 (Mann–Whitney)

    Article Snippet: To conduct immunoprecipitation, a total of 2 µg of goat anti-mouse CD36 antibody (R&D) or normal goat IgG (Millipore) was incubated with Pierce™ Protein A/G Magnetic Beads (Thermo Scientific™) overnight.

    Techniques: Immunoprecipitation, Mass Spectrometry, Staining, Confocal Microscopy, Western Blot, Derivative Assay, Positive Control, Negative Control, Functional Assay, MANN-WHITNEY

    The protein interaction between CD36 and FcγRIIb was confirmed through various experimental approaches. A CD36 and FcγRIIb staining analysis of CPG-stimulated B cells using confocal microscopy. B The proteins immunoprecipitated by anti-CD36 antibodies were detected using anti-FcγRIIb antibodies in B cells stimulated with cytidine phosphate guanosine (CPG) or lipopolysaccharide (LPS). The total B cell protein served as the loading (positive) control referred to as Input (Inp). The B cell protein was immunoprecipitated using isotype (negative) control IgG antibodies (IgG) or anti-CD36 antibodies (CD36). C , D Docking scores and ligand mean square deviation (rmsd) for the models of CD36 docking with FcγRIIb by HDOCK SERVER. The X-axis represents the rank of the top 10 models, and the Y-axis shows the docking energy scores in C . The ligand rmsd is calculated by comparing the ligands in the CD36 model with the modeled FcγRIIb structures in D . E , F The front and back sides of the model 1 of CD36 (multicolor) docking with FcγRIIb (purple)

    Journal: Cellular & Molecular Biology Letters

    Article Title: Unveiling the hidden role of the interaction between CD36 and FcγRIIb: implications for autoimmune disorders

    doi: 10.1186/s11658-024-00593-7

    Figure Lengend Snippet: The protein interaction between CD36 and FcγRIIb was confirmed through various experimental approaches. A CD36 and FcγRIIb staining analysis of CPG-stimulated B cells using confocal microscopy. B The proteins immunoprecipitated by anti-CD36 antibodies were detected using anti-FcγRIIb antibodies in B cells stimulated with cytidine phosphate guanosine (CPG) or lipopolysaccharide (LPS). The total B cell protein served as the loading (positive) control referred to as Input (Inp). The B cell protein was immunoprecipitated using isotype (negative) control IgG antibodies (IgG) or anti-CD36 antibodies (CD36). C , D Docking scores and ligand mean square deviation (rmsd) for the models of CD36 docking with FcγRIIb by HDOCK SERVER. The X-axis represents the rank of the top 10 models, and the Y-axis shows the docking energy scores in C . The ligand rmsd is calculated by comparing the ligands in the CD36 model with the modeled FcγRIIb structures in D . E , F The front and back sides of the model 1 of CD36 (multicolor) docking with FcγRIIb (purple)

    Article Snippet: To conduct immunoprecipitation, a total of 2 µg of goat anti-mouse CD36 antibody (R&D) or normal goat IgG (Millipore) was incubated with Pierce™ Protein A/G Magnetic Beads (Thermo Scientific™) overnight.

    Techniques: Staining, Confocal Microscopy, Immunoprecipitation, Positive Control, Negative Control

    Complex template information for CD36 and FcγRIIb

    Journal: Cellular & Molecular Biology Letters

    Article Title: Unveiling the hidden role of the interaction between CD36 and FcγRIIb: implications for autoimmune disorders

    doi: 10.1186/s11658-024-00593-7

    Figure Lengend Snippet: Complex template information for CD36 and FcγRIIb

    Article Snippet: To conduct immunoprecipitation, a total of 2 µg of goat anti-mouse CD36 antibody (R&D) or normal goat IgG (Millipore) was incubated with Pierce™ Protein A/G Magnetic Beads (Thermo Scientific™) overnight.

    Techniques:

    Interface residue pairs and ligand root mean square deviation (rmsd) (Å) for Model 1 of CD36 docking with FcγRIIb

    Journal: Cellular & Molecular Biology Letters

    Article Title: Unveiling the hidden role of the interaction between CD36 and FcγRIIb: implications for autoimmune disorders

    doi: 10.1186/s11658-024-00593-7

    Figure Lengend Snippet: Interface residue pairs and ligand root mean square deviation (rmsd) (Å) for Model 1 of CD36 docking with FcγRIIb

    Article Snippet: To conduct immunoprecipitation, a total of 2 µg of goat anti-mouse CD36 antibody (R&D) or normal goat IgG (Millipore) was incubated with Pierce™ Protein A/G Magnetic Beads (Thermo Scientific™) overnight.

    Techniques: Residue

    Loss of FcγRIIb in mice downregulates CD36 expression in B cells. A The gating strategy and scatter plot depict CD36 staining in marginal zone B cells (MZB) (CD19 + CD21 + CD23 mid ) from both wild-type (WT) and Fcgr2b knockout ( Fcgr2b KO) mice determined via flow cytometry. B The immunization strategy involved apoptotic cells (4xAC) in WT and Fcgr2b KO mice. PBS served as the negative control. C The CD36 staining was performed on MZB in WT and Fcgr2b KO mice under injections. D , E The gating strategy and scatter plot illustrate the CD36 staining pattern in germinal center B cells (GC) (B220 + IgD − CD95 + GL-7 + ) of and plasma cells (PC) (B220 int CD138 + ) in immunized WT and Fcgr2b KO mice. F The FcγRIIb staining on B cells, follicular B cells (FOB), and marginal zone B cells (MZB). G The immunization strategy was used for both WT and Cd36 knockout ( Cd36 KO) mice with four times weekly apoptotic cells (4xAC) or PBS. H The FcγRIIb staining of MZB in WT and Cd36 KO mice after treatments. The data are representative of three independent experiments. * P < 0.05, ** P < 0.01 and *** P < 0.001 (Mann–Whitney)

    Journal: Cellular & Molecular Biology Letters

    Article Title: Unveiling the hidden role of the interaction between CD36 and FcγRIIb: implications for autoimmune disorders

    doi: 10.1186/s11658-024-00593-7

    Figure Lengend Snippet: Loss of FcγRIIb in mice downregulates CD36 expression in B cells. A The gating strategy and scatter plot depict CD36 staining in marginal zone B cells (MZB) (CD19 + CD21 + CD23 mid ) from both wild-type (WT) and Fcgr2b knockout ( Fcgr2b KO) mice determined via flow cytometry. B The immunization strategy involved apoptotic cells (4xAC) in WT and Fcgr2b KO mice. PBS served as the negative control. C The CD36 staining was performed on MZB in WT and Fcgr2b KO mice under injections. D , E The gating strategy and scatter plot illustrate the CD36 staining pattern in germinal center B cells (GC) (B220 + IgD − CD95 + GL-7 + ) of and plasma cells (PC) (B220 int CD138 + ) in immunized WT and Fcgr2b KO mice. F The FcγRIIb staining on B cells, follicular B cells (FOB), and marginal zone B cells (MZB). G The immunization strategy was used for both WT and Cd36 knockout ( Cd36 KO) mice with four times weekly apoptotic cells (4xAC) or PBS. H The FcγRIIb staining of MZB in WT and Cd36 KO mice after treatments. The data are representative of three independent experiments. * P < 0.05, ** P < 0.01 and *** P < 0.001 (Mann–Whitney)

    Article Snippet: To conduct immunoprecipitation, a total of 2 µg of goat anti-mouse CD36 antibody (R&D) or normal goat IgG (Millipore) was incubated with Pierce™ Protein A/G Magnetic Beads (Thermo Scientific™) overnight.

    Techniques: Expressing, Staining, Knock-Out, Flow Cytometry, Negative Control, MANN-WHITNEY